Macroscopic X-ray fluorescence spectrometry (MA-XRF) imaging technique enables non-destructive elemental mapping of large surface areas. It has a wide application in the analytical examination of works of art, through the identification of the distribution of inorganic chemical elements on the painted surface. In July 2023, under the auspices of the National Gallery of Athens, MA-XRF measurements were carried out on the painting “30 March 1814” by Muller Charles-Louis-Lucien.